Fig. 1.

Effect of γ-radiation in normal human fibroblast cells. (A) MRC-5 cells were incubated for 48 h after exposure to γ-radiation as indicated. Cell viability was determined by MTT assay, and data were expressed as means ± SD (*P < 0.05 and **P < 0.005 compared with untreated controls). (B) Cells were prepared at 8 h after exposure to γ-irradiation (0, 0.05 and 2 Gy), and expression levels of phospho-p53 (Ser15), p53 and p21 were determined by western blot analysis. β-actin was used as a loading control. (C) Cell viability was determined by MTT assay as shown Fig. 1A in NHDF cells. Data represent three independent experiments expressed as means ± SD (*P < 0.05 and **P < 0.005 compared with untreated controls). (D) Western blotting was performed as indicated in Fig. 1B in NHDF cells.