Fig. 2.

Differences in phosphoprotein profiles in response to low-dose and high-dose radiation in MRC-5 cells. MRC-5 cells were treated with 0.05 and 2 Gy γ-radiation as indicated, and cell lysates were extracted from MRC-5 cells after 0.5 h. Early response phosphoproteins were identified based on ≥1.1-fold changes from three independent experiments. (A) Representative Venn diagram shows the numbers of phosphoproteins identified in antibody microarrays that were upregulated ≥1.1-fold in response to γ-radiation as indicated at the time and dose ranges. (B) Distribution of protein class of biological functions (GO processes) for upregulated phosphoproteins (≥1.1-fold) associated with the early response (0.5 h) to γ-radiation. In t-tests, all changes were significant (P < 0.05).