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. 2017 Jan 25;58(3):329–340. doi: 10.1093/jrr/rrw126

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© The Author 2017. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 3. — Antibody microarray analysis validates phosphoproteins involved in the early response to γ-radiation. (A and B) Normal human fibroblast cells were incubated for 0.5 h after exposure to 0.05 or 2 Gy of γ-radiation. Expression of levels of phospho-NBS1 (Ser343), NBS1, phospho-Gab2 (Tyr643), Gab2, phospho-BTK (Tyr550), BTK, phospho-ETK (Tyr40), ETK, phospho-Elk1 (Ser383), Elk1, phospho-CamK4 (Thr196/200) and CamK4 were determined by western blotting. (A, MRC-5 cells; B, NHDF cells). β-actin was used as a loading control. The asterisk (*) indicates nonspecific band.