Table 2.
Phosphoproteins responding to high-dose radiation (2 Gy) in human fibroblast cells
| Phosphoprotein name | Microarray ratio | P-value | Swiss prot. |
|---|---|---|---|
| Mean ± SD | Accession no. | ||
| Phospho-MEK1 (Thr298) | (+) 1.570 ± 0.58 | 0.043 | Q02750 |
| Phospho-PLCG1 (Tyr1253) | (+) 1.558 ± 0.15 | 0.003 | P19174 |
| Phospho-IRS-1 (Ser612) | (+) 1.522 ± 0.32 | 0.015 | P35568 |
| Phospho-TFII-I (Tyr248) | (+) 1.519 ± 0.36 | 0.019 | P78347 |
| Phospho-IKK-alpha/beta (Ser176/177) | (+) 1.461 ± 0.53 | 0.042 | O15111 |
| Phospho-MEK1 (Thr286) | (+) 1.432 ± 0.48 | 0.036 | Q02750 |
| Phospho-Pyk2 (Tyr580) | (+) 1.405 ± 0.25 | 0.011 | Q14289 |
| Phospho-Keratin 8 (Ser431) | (+) 1.397 ± 0.53 | 0.045 | P05787 |
| Phospho-ERK3 (Ser189) | (+) 1.360 ± 0.31 | 0.017 | Q16659 |
| Phospho-Chk1 (Ser296) | (+) 1.322 ± 0.61 | 0.007 | O14757 |
| Phospho-CBL (Tyr700) | (+) 1.318 ± 0.02 | 0.000 | P22681 |
| Phospho-BTK (Tyr550) | (+) 1.316 ± 0.20 | 0.008 | Q06187 |
| Phospho-LIMK1/2 (Thr508/505) | (+) 1.302 ± 0.38 | 0.027 | P53667 |
The Student's t-test indicated that average fold-change values were significant at the level of P < 0.05; (+) indicates increased protein expression in MRC-5 cells; SD indicates standard deviation of fold-change in triplicate independent experiments.