TABLE 1.
Mutations in clone C compared to swine HEV consensus sequence and correction of the mutations in three derived clones (pSHEV-1, pSHEV-2, and pSHEV-3)
| Fragment | Genomic region | Clone C mutationa
|
Correction of mutation
|
|||
|---|---|---|---|---|---|---|
| nt | aa | pSHEV-1 | pSHEV-2 | pSHEV-3 | ||
| HB | Methyl domainb | T38Ac | Silent | |||
| BX | Y domain | A756-(deletion) | Frameshift | A | A | A |
| Y domain | C819T | Silent | C | C | C | |
| Y domain | T932C | Silent | T | T | T | |
| Y domain | C1028Ac | Silent | ||||
| XAf | X domain | T2765C | Silent | T | ||
| RdRp | C4085T | Silent | C | |||
| RdRp | T4175A | Silent | T | |||
| RdRp | T4307C | Silent | T | |||
| AvN | ORF2 | T5338C | F51L | T | T | |
| ORF2 | A5362G | T59A | A | A | ||
| ORF2 | T5811C | Silent | T | T | ||
| ORF2 | C6252T | Silent | C | C | ||
| ORF2 | C6356T | S390L | C | C | ||
a
Mutation in clone C compared to the swine HEV consensus sequence. The nucleotide or amino acid residue prior to the position number represents the consensus sequence.
b
Methyl domain, methyltransferase domain.
c
Mutations at nucleotide positions 38 and 1028 in all three cDNA clones were not corrected and were used as genetic markers.