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Sensitivity of R5X4 and R5 strains to CCR5 blocker M657. U87/CD4/CCR5 cells were treated for 1 h with different concentrations of M657 and infected with prototype and primary R5X4 (89.6, DH12, 93BR020, and 92HT594) and R5 strains (Bal, SF162, and BL2). Two days later cells were washed and lysed, and cellular DNA was subjected to quantitative real-time PCR amplification to detect HIV-1 LTR or cellular GAPDH sequences. The experiment was repeated three times with similar results.
