TABLE 2.
Syncytium formation over time by mutant NBV p10s with mutations at hydrophilic or charged residuesa
| Construct | Syncytium formationb at:
|
|||
|---|---|---|---|---|
| 24 h | 36 h | 48 h | 72 h | |
| NBV p10 | ++++ | D | D | D |
| S10G | +++ | ++++ | D | D |
| S10V | ++ | ++ | +++ | ++++ |
| S14G | ++ | ++ | +++ | +++ |
| S14V | + | ++ | +++ | +++ |
| H16A | +++ | ++++ | D | D |
| H16D | +++ | ++++ | D | D |
| Mock | − | − | − | − |
Gly and Val were introduced at residues 10-Ser and 14-Ser. The charged residue 16-His was mutated to Ala or Asp as described in Materials and Methods.
See Table 1, footnote b, for explanation of the results. The results shown are representative of three separate experiments.