TABLE 3.
Recombination between two markers separated by 300 bp
Accessory gene products present and cell line | Total no. of live events | No. of infected cells | No. of GFP+ cells | Infection MOI | GFP MOI | GFP MOI/ infection MOI | % of TMMRRa |
---|---|---|---|---|---|---|---|
With all accessory gene products | |||||||
Cell line 1 | 169,742 | 68,118 | 4,751 | 0.51 | 0.028 | 0.055 | 44.3 |
Cell line 2 | 457,070 | 125,496 | 6,071 | 0.32 | 0.013 | 0.042 | 33.3 |
Cell line 3 | 377,710 | 142,939 | 8,228 | 0.48 | 0.022 | 0.046 | 37.1 |
Mean ± SD | 38.2 ± 5.6 | ||||||
Without Vif, Vpr, Vpu, and Nef | |||||||
Cell line 1 | 177,088 | 43,332 | 2,513 | 0.28 | 0.014 | 0.051 | 40.7 |
Cell line 2 | 543,741 | 133,692 | 6,768 | 0.28 | 0.013 | 0.044 | 35.5 |
Cell line 3 | 710,696 | 157,916 | 7,463 | 0.25 | 0.011 | 0.042 | 33.6 |
Mean ± SD | 36.6 ± 3.7 |
TMMRR, theoretical maximum measurable recombination rate.