TABLE 4.
Recombination between two markers separated by 288 bp
Accessory gene products present and cell line | Total no. of live events | No. of infected cells | No. of GFP+ cells | Infection MOI | GFP MOI | GFP MOI/ infection MOI | % of TMMRRa |
---|---|---|---|---|---|---|---|
With all accessory gene products | |||||||
Cell line 1 | 174,404 | 72,866 | 4,140 | 0.54 | 0.024 | 0.044 | 35.5 |
Cell line 2 | 540,508 | 195,323 | 8,225 | 0.45 | 0.015 | 0.034 | 27.4 |
Cell line 3 | 271,641 | 127,694 | 6,218 | 0.64 | 0.023 | 0.036 | 29.2 |
Mean ± SD | 30.7 ± 4.3 | ||||||
Without Vif, Vpr, Vpu, and Nef | |||||||
Cell line 1 | 176,526 | 41,014 | 1,777 | 0.26 | 0.010 | 0.038 | 30.6 |
Cell line 2 | 469,566 | 144,843 | 6,371 | 0.37 | 0.014 | 0.037 | 29.6 |
Cell line 3 | 585,638 | 203,767 | 8,356 | 0.43 | 0.014 | 0.034 | 26.9 |
Mean ± SD | 29.0 ± 1.9 |
TMMRR, theoretical maximum measurable recombination rate.