TABLE 5.
Recombination between two markers separated by 103 bp
| Accessory gene products present and cell line | Total no. of live events | No. of infected cells | No. of GFP+ cells | Infection MOI | GFP MOI | GFP MOI/ infection MOI | % of TMMRRa |
|---|---|---|---|---|---|---|---|
| With all accessory gene products | |||||||
| Cell line 1 | 155,972 | 63,480 | 1,455 | 0.52 | 0.009 | 0.018 | 14.3 |
| Cell line 2 | 1,067,637 | 405,475 | 6,241 | 0.48 | 0.006 | 0.012 | 9.8 |
| Cell line 3 | 1,049,218 | 321,544 | 5,021 | 0.37 | 0.005 | 0.013 | 10.5 |
| Mean ± SD | 11.6 ± 2.4 | ||||||
| Without Vif, Vpr, Vpu, and Nef | |||||||
| Cell line 1 | 171,884 | 44,695 | 841 | 0.30 | 0.005 | 0.016 | 13.0 |
| Cell line 2 | 1,094,731 | 387,098 | 5,960 | 0.44 | 0.005 | 0.013 | 10.0 |
| Cell line 3 | 1,319,480 | 372,408 | 5,565 | 0.33 | 0.004 | 0.013 | 10.2 |
| Mean ± SD | 11.1 ± 1.7 |
TMMRR, theoretical maximum measurable recombination rate.