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. 2017 May 22;17:360. doi: 10.1186/s12885-017-3330-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of ENDOD1 on cell cycle and apoptosis. a-b cell cycle distribution without or with overexpression of ENDOD1 in DU145 cells. Overexpression of ENDOD1 led to notable increases in G0/G1 phase arrest, with a corresponding decrease in the percentage of cells in the G2/M and S phases. c-d cell cycle distribution after transfecting siRNA or si-NC of ENDOD1 in LNCaP cells. siRNA-induced downregulation of ENDON1 led to remarkable increase in the percentage of cells in the G2/M phase. e-f FITC-Anexin V test demonstrated no changes in apoptosis between the control group and the group of ENDOD1 overexpression (e) or siRNA (f). Error bars represent the means of three independent experiments. *P <0.05.