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Localization of HA-tagged 2Apro in PV-infected neural cells. Neural cells infected with 2A-HA virus were cultured as described in Materials and Methods. The cells were not treated 2 hpi with MAb (A, D, and G) or were treated 2 hpi with MAb against PV (B, E, and H) or against hPVR (C, F, and I). The cells were fixed 5 hpi (A, B, and C) or 11 hpi (D, E, F, G, H, and I) and subjected to an immunofluorescence study. Red indicates nucleic acids, and green indicates HA-tagged 2Apro.
