Transient expression of HA-tagged 2Apro. Mammalian expression vector pCI-neo encoding HA-tagged 2Apro was transfected into cells, which were subjected to an immunofluorescence study. Neural cells (A, B, C, and D) and HeLa cells (E, F, G, and H) were fixed 2 days posttransfection. Red indicates nucleic acids, and green indicates HA-tagged 2Apro.