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. 2017 May 22;18:268. doi: 10.1186/s12859-017-1684-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Anisotropic image enhancement. a, c Images of U2OS cell plated on crossbow-shaped micropattern before (A) and after (C) image enhancement by LFT and OFT (B). Blue box: a region containing two parallel filaments of low contrast with background. Purple box: an area containing a cluster-like noise and a filamentous structure. b An illustrative enhancement filter with a total length of 2r and a stepwise rotation angle of θ. Scale bar, 5 μm. d, e Enlarged images of blue and purple boxes in (A) and (B) respectively. Scale bar, 1 μm. f Normalized intensity profiles of the green-cropped regions (D, E, left) the blue square boxes from (A) and (B). g Normalized intensity profiles of the red-cropped regions (D, E, right) in the purple square boxes from (A) and (B).