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. 2017 May 22;18:268. doi: 10.1186/s12859-017-1684-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Stress fiber extraction and orientations. a Skeleton of stress fibers (red) overlaid on TRFM image of the protrusive region of U2OS cell plated on crossbow-shaped micropattern. b, c Enlarged regions of green and blue boxes in (A). d Binary image of stress fiber network overlaid with network skeleton (red). e Distribution of actin orientations. Colorbar, degree