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. 2016 Sep 10;5:262–273. doi: 10.1016/j.bonr.2016.09.001

Fig. 6.

Fig. 6

Mineralized nodules formation of MC3T3-E1 cells cultured with osteogenic medium. (A) Representative images of mineralized nodules stained by Alizarin Red S, (a) DMEM control, (b) DRTF (12.5 μg/ml) treatment, bar: 100 μm. (B) Quantitative analysis of the area of mineralized nodules using Image J software. Values are mean ± SD (n = 3), **p < 0.01 vs. control.