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Effects of DRTF on the OPG and RANKL protein levels in MC3T3-E1 cells. (A) Western blot analysis of OPG and RANKL expression after treatment of MC3T3-E1 cells with E2 or DRTF (12.5 μm/ml) for 48 h. (B) “Quantity one” from Bio-Rad was used to analyze the western blot results, the values represent mean ± SD (n = 3), *p < 0.05, **p < 0.01 vs. control.
