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Effects of DRTF on ERα and ERβ mRNA expression in MC3T3-E1 cells. MC3T3-E1 cells were treated with vehicle (control), E2 and different concentrations of DRTF (2.5, 12.5 and 62.5 μg/ml) for 12 h. Total RNA was isolated and real-time RT-PCR was performed to determine the mRNA expressions levers of ERα (A) and ERβ (B), which were normalized to that of GAPDH. The results were expressed as the mean ± SD (n = 3), *p < 0.05, **p < 0.01 vs. control.
