. 2016 Sep 10;5:262–273. doi: 10.1016/j.bonr.2016.09.001

Table 1.

The primer sequences of real time RT-PCR.

Gene	GeneBank no.	Primer sequences	Annealing temperature (°C)	Product length (bp)
OPG	NM_008764.3	Forward: 5′-CCAAAGTGAATGCCGAGAGT-3′	57	154
OPG	NM_008764.3	Reverse: 5′-ACGCTGCTTTCACAGAGGTC-3′	57	154
Rankl	NM_011613.3	Forward: 5′-CTCACCATCAATGCTGCCAG-3′	58	162
Rankl	NM_011613.3	Reverse: 5′-GCAAATGTTGGCGTACAGGT-3′	58	162
ERα	NM_001302533.1	Forward: 5′-TGAAAGGCGGCATACGGAAA-3′	60	105
ERα	NM_001302533.1	Reverse: 5′-TGTCTCCTGAAGCACCCATT-3′	60	105
ERβ	NM_010157.3	Forward: 5′-TAGAGAGCCGTCACGAATAC-3′	58	161
ERβ	NM_010157.3	Reverse: 5′-GGCTAAAGGAGAGAGGTGTC-3′	58	161
GAPDH	NM_008084.2	Forward: 5′-AGGGCATCTTGGGCTACACT-3′	58	161
GAPDH	NM_008084.2	Reverse: 5′-CACCCTGTTGCTGTAGCCGT-3′	58	161