TABLE 1.
Screening of STAR cells expressing RRV and SFV Enva
| Screening (time point) | No. of vector-producing clones/total clones (eGFP titer [IU/ml]) for
|
||
|---|---|---|---|
| MLV-A | RRV | SFV | |
| 1 (5 weeks) | 22/48 | 16/48 | 11/48 |
| 2 (11 weeks) | 19/22 (5 × 105 ∼ 2 × 107) | 6/16 (105 ∼ 106) | 3/11 (5 × 103 ∼ 2 × 104) |
| 3 (20 weeks) | 19/19 (5 × 105 ∼ 2 × 107) | 5/6 (105 ∼ 4 × 105) | 3/3 (5 × 103 ∼ 2 × 104) |
a
Infectious vector production from phleomycin-resistant clones was monitored for up to 5 months. Vector production was examined by inoculating the culture supernatants of the clones on 293T cells in the presence of 8 μg of Polybrene/ml at the indicated time points.