TABLE 4.
Infection of murine and human primary cell culturesa
| Envelope | MOI | % Infected cells (±SD)
|
|||
|---|---|---|---|---|---|
| mDC (B6) | mDC (B/c) | hDC | hPBL | ||
| RRV | 3 | 14.3 ± 4.2 | 13.4 ± 1.4 | 1.9 ± 1.1 | <0.1 |
| MLV-A | 32 | 2.0 ± 0.3 | 1.6 ± 0.6 | 1.9 ± 0.1 | 26.5 ± 6.4 |
| MLV-A | 3 | 0.8 ± 0.2 | 0.5 ± 0.2 | 0.3 ± 0.1 | 6.8 ± 2.8 |
| VSV-G | 24 | 53.9 ± 12.7 | 38.5 ± 2.4 | 18.7 ± 11.2 | 7.1 ± 0.3 |
| VSV-G | 3 | 8.4 ± 2.1 | 5.9 ± 0.6 | 6.3 ± 0.8 | 3.5 ± 1.0 |
a
Primary cell cultures of murine DC (mDC) from BALB/c (B/c) and C57BL/6 (B6) mice, human DC (hDC), and human peripheral blood lymphocytes (hPBL) were infected with the indicated pseudotypes. Shown in the table is the percentage of cells infected. Vector inputs were adjusted to the same MOI (MOI, 3) or to the same volume [MOI of 32 for HIV-1(MLV-A) and MOI of 24 for HIV-1 (VSV-G)]. MOI was determined by titration of pseudotypes onto 293T cells in the presence of 8 μg of Polybrene/ml in parallel with primary cell culture infection.