TABLE 2.
Competition analysis of Sin Nombre virus GST-N protein-RNA interactionsa
| Competitor RNA | Labeled RNA bound to N (% of wild-type value) |
|---|---|
| Wild-type S RNA | 10 ± 1 |
| RNA 14 | 81 ± 3 |
| RNA 3 | 63 ± 2 |
| RNA 2 | 71 ± 4 |
| RNA 12 | 15 ± 2 |
| RNA 13 | 59 ± 5 |
| RNA 15 | 80 ± 6 |
| RNA 8 | 18 ± 1 |
| RNA 7 | 35 ± 2 |
| RNA 10 | 19 ± 1 |
| RNA 9 | 37 ± 3 |
| RNA 11 | 59 ± 5 |
| RNA 5 | 21 ± 1 |
| RNA 6 | 73 ± 4 |
| RNA 17 | 23 ± 2 |
| RNA 18 | 19 ± 1 |
| RNA 16 | 20 ± 3 |
Increasing unlabeled RNAs were used in competition with 32P-labeled wild-type RNA as in Fig. 3. The ability of each RNA to compete for binding to trimeric N is indicated by the maximal reduction in the binding of 32P-labeled wild-type RNA. The results are based on three independent experiments.