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. 2005 Feb;79(3):1824–1835. doi: 10.1128/JVI.79.3.1824-1835.2005

TABLE 2.

Competition analysis of Sin Nombre virus GST-N protein-RNA interactionsa

Competitor RNA Labeled RNA bound to N (% of wild-type value)
Wild-type S RNA 10 ± 1
RNA 14 81 ± 3
RNA 3 63 ± 2
RNA 2 71 ± 4
RNA 12 15 ± 2
RNA 13 59 ± 5
RNA 15 80 ± 6
RNA 8 18 ± 1
RNA 7 35 ± 2
RNA 10 19 ± 1
RNA 9 37 ± 3
RNA 11 59 ± 5
RNA 5 21 ± 1
RNA 6 73 ± 4
RNA 17 23 ± 2
RNA 18 19 ± 1
RNA 16 20 ± 3
a

Increasing unlabeled RNAs were used in competition with 32P-labeled wild-type RNA as in Fig. 3. The ability of each RNA to compete for binding to trimeric N is indicated by the maximal reduction in the binding of 32P-labeled wild-type RNA. The results are based on three independent experiments.