Fig. 2.

TanIIA reduced CXCL12-induced A375 cell invasive ability in a dose dependent manner. To test the effects of Tan II A on melanoma cell invasion, A375 cells were incubated with TanIIA at 0, 1, 2, or 4 μg/mL for 24 or 48 h. The tested cells were trypsin digested, counted, washed, and adjusted to 0.6 × 10⁶ cell/ml. The treated cells were placed in top chamber of transwell with 10 ng/ml chemokine CXCL12 for 24 h to induce the cell invasion. The A375 cells traveled to the bottom chamber were then stained with CeU Counting Kit-8 (CCK-8). The cell image and number were recorded and counted. a. The images of invasive A375 cells in the bottom chamber under a microscope (200 X amplification). b. Tan II A significantly decreased A375 cells invasive ability in a dose dependent manner. Each treatment was repeated three times (n = 3). *P < 0.05, ** P < 0.01, and ***P < 0.001 for comparison of indicated treatments to the negative control (0 μg/mL) at 24 or 48 h respectively