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. 2017 May 22;16:22. doi: 10.1186/s40200-017-0303-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Gel electrophoresis showing PCR- RFLP analysis of MTHFR, C677T locus; rs1801133. Detection of C677T polymorphism after HinfI digestion: fragments in lanes 1, 5, and 10 (171bp, 74 bp) indicate homozygote (CC) genotype; 3,4, 6, 7, 8, and 9 (245 bp) lanes 1, 5 and 10 showing a single band (245 bp) of undigested PCR product indicates homozygote (TT) genotype; lanes 2,and 11 heterozygote (C/T) genotype (245, 171 and 74 bp). L; ladder 100bp, gel 2%