FIG. 6.
HCV replicon induces cyclin D1 and Bcl-XL expression. (A) Huh-7 and FCA4 cells were transiently transfected with cyclin D1 luciferase reporter along with the dominant negative mutants of Src (pM5Hmet295), JNK (p54JNK2α), and STAT-3 (pGS5hSTAT-3β; STAT-3S727A). At 36 h posttransfection, cellular lysates were prepared for luciferase activity assays. (B) Equal amounts of Huh-7 and FCA4 lysates expressing dominant negative mutants of STAT-3 were subjected to SDS-PAGE and immunoblotted with anti-Bcl-XL serum. Lane 1, Huh-7 lysates; lane 2, FCA4 lysates; lanes 3 and 4, FCA4 lysates expressing STAT-3 dominant negative mutants.