FIG. 8.

HCV replicon induces intracellular production of ROS. (A) Huh-7 and FCA4 cells were treated with 4 μM DHE for 45 min. Cells were harvested, and ROS levels were measured by flow cytometry with excitation emission at 605 nm. The bars show the fold increase in oxidized DHE fluorescence. (B) Untransfected (control) or in vitro-synthesized BM4-5 RNA-transfected Huh-7 cells were treated with 4 μM DHE. ROS levels were assessed in untransfected cells (blue) and cells transfected with BM4-5 RNA (red).