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. 2017 May 22;16:61. doi: 10.1186/s12938-017-0350-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — A sample MRI image at L4–L5 and the processed image after each step of the algorithm; a the original MRI image, b adaptive equalized algorithm image, c adjusted contrast image, d the select area, e the cropped area of the selected ROI, f the resulted binary image from automated algorithm (left) and manual thresholding technique (right)