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. 2017 May 22;13:41. doi: 10.1186/s13007-017-0191-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — PCR products of chi26 (800 bp) (a) and bar (400 bp) (b) genes from the 14 independent T0 transgenic lines (CHI 1, CHI 3, CHI 6, CHI 7, CHI 9, CHI 10, CHI 14, CHI 16, CHI 20, CHI 22, CHI 30, CHI 47, CHI 50, and CHI 71, lanes 1–14, respectively). M: Quick-Load^® 100 bp DNA Ladder (New England Biolabs), +: positive control (pBarley/chi/bar), −: negative control (non-transgenic, cv. Hi-Line)