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. 2017 May 22;13:41. doi: 10.1186/s13007-017-0191-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Southern blot analysis of the five independent chi26 transgenic plants (CHI 7, CHI 14, CHI 47, and CHI 71, respectively) at T0 generation. Genomic DNA of each line was digested with NcoI and fragments were resolved by 0.8% agarose gel electrophoresis. The blot was probed by a BamHI fragment involving a maize ubi promoter and maize ubi intron 1 (~2 kb). M; 1 Kb DNA ladder (New England Biolabs) with 0.5, 1, 1.5, 2, 3, 4, 5, 6, 8 and 10 kb, –: non-transgenic cv. Hi-Line