Fig. 5.

Impact of single vs. dual MET/PI3K inhibition on downstream signaling and cell proliferation in non-MET-driven HNC models. a, cells were serum-starved for 24 h, treated with tepotinib for 2 h, and then stimulated with the MET ligand SF/HGF for 10 min. b, cells were treated for 16 h with half-EC₅₀ concentrations of tepotinib and pictilisib, alone and in combination, and then lysed. c, cells were treated for 72 h with single or dual MET/PI3K inhibitors at various concentrations. Cell proliferation was assessed using a resazurin-reduction based assay and combinatorial effects were determined using the excess over bliss, with values above 0 indicating more-than-additive effects (bold-italic numbers indicate values significantly different to 0 according to one-sample t-test)