TABLE 1.
Rate constants and equilibrium constants for the binding of the different constructs to 17b as a function of pH as measured by BIAcore SPR
| Protein | Valuea at:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| pH 9
|
pH 7
|
pH 5
|
|||||||
| ka (M−1 s−1) (10−5) | kd (s−1) (104) | KD (M) (109) | ka (M−1 s−1) (10−5) | kd (s−1) (104) | KD (M) (109) | ka (M−1 s−1) (10−5) | kd (s−1) (104) | KD (M) (109) | |
| gp120:CD4D12 (1:5) | 1.7 | 5.6 | 3.4 | 1.8 | 3.0 | 1.6 | 3.1 | 4.6 | 1.5 |
| gp120-CD4D12 | 2.2 | 2.2 | 1.0 | 3.0 | 2.3 | 0.8 | 2.2 | 4.9 | 2.2 |
| gp120-M9 | 0.2 | 6.0 | 32.0 | 0.3 | 7.9 | 25.0 | 0.2 | 4.5 | 20 |
a
ka and kd, rate constants for association and dissociation, respectively. KD, equilibrium dissociation constant.