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Characterization of neutralization properties of guinea pig sera before and after affinity purification on immobilized gp120-M9 beads
| Serial no. | Material | ELISA titer
|
Neutralization titer
|
||
|---|---|---|---|---|---|
| CD4a | gp120a | MNb | 1357-0b | ||
| 1 | Untreated sera | 1,000,000 | 150,000 | 640 | 80 |
| 2 | Column flowthrough | 1,000,000 | 150,000 | 704 | 88 |
| 3 | Eluted fraction | 3,600 | 150,000 | <44 | <44 |
ELISA antigen.
Viral strain used for neutralization assay.
