Table 2.
gB2, gB3 peptides, and non-cleaved peptide (NCP) and their respective kcat, and km kinetics parameters.
| Peptide | Mutation | kcat (1/s) | SD | km (μmolar) | SD | kcat/km | Sequence name | Genotype |
|---|---|---|---|---|---|---|---|---|
| RTRR↓STSDNNT | – | 2.24 | 0.05 | 3.19 | 0.06 | 0.70 | gB2_13 | 2 |
| RTRR↓SRSDNNT | T462R | 5.06 | 0.08 | 0.23 | 0.01 | 22.00 | gB2_19 | |
| RTRR↓SMSDNNT | T462M | 3.01 | 0.29 | 1.65 | 0.14 | 1.82 | gB2_18 | |
| RTRR↓SASDNNT | T462A | 4.06 | 0.03 | 0.69 | 0.04 | 5.88 | gB2_20 | |
| RTKR↓STGNTTT | – | 0.25 | 0.02 | 0.02 | 0.01 | 12.5 | gB3_22 | 3 |
| RTKR↓DTGNTTT | S461D | 1.89 | 0.11 | 0.43 | 0.02 | 4.39 | gB3_26 | |
| ATRASTSDNNT | R457A/R460A | – | – | – | – | – | – | NCP |
Standard deviation (SD) were calculated and shown for each parameter.