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. 2017 Apr 10;13(5):561–573. doi: 10.7150/ijbs.19032

Figure 2.

Figure 2

The Wnt/β-Catenin signaling pathway was activated in human osteosarcoma cells. (A) Elevated protein levels of members of the Wnt/β-Catenin signaling pathway. Protein levels of FOXM1, β-Catenin, c-Myc, Cyclin D1 and GAPDH in hFOB1.19, U2OS, MG63, Saos-2 and HOS cells were determined by western blot. (B) Statistical analysis of the protein levels in (A). (C) Wnt3a treatment increased the levels of proteins of the Wnt/β-Catenin signaling pathway. Protein levels of β-Catenin, FOXM1, c-Myc, Cyclin D1 and GAPDH in U2OS cells upon the treatment of Wnt3a (20 ng/ml) for 0, 30, 60 and 90 min, were determined by western blot. (D) Statistical analysis of the protein levels in (C). Representative data from three independent experiments are shown. **P<0.001.