Figure 3.
Knockdown of FOXM1 or β-Catenin inhibited osteosarcoma cell growth. Knockdown of FOXM1 or β-Catenin in hFOB1.19, U2OS and MG63 cells resulted in attenuated expression of c-Myc and Cyclin D1. The hFOB1.19, U2OS or MG63 cells transfected with control-shRNA, FOXM1-shRNA or β-Catenin-shRNA were lysed and probed with anti-FOXM1, β-Catenin, c-Myc, Cyclin D1 or GAPDH antibody. (B) Knockdown of FOXM1 or β-Catenin in hFOB1.19, U2OS and MG63 cells inhibited cell proliferation. Cells used in (A) were subjected to the MTT assay to evaluate cell proliferation, and the cell viability was determined at 490 nm. (C-D) Knockdown of FOXM1 or β-Catenin in hFOB1.19, U2OS and MG63 cells decreased the rate of colony formation. Cells (1 x 103) used in (A) were seeded onto 6-well plates, and cultured with 0.1 ml DMEM medium for two weeks. Then, cells were stained with 0.5% crystal violet (D), and the number of colonies was counted (E). Representative data from three independent experiments are shown. **P<0.001.