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. 2017 May 15;13(5):630–639. doi: 10.7150/ijbs.18485

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Blebbistatin inhibits GSK3β expression in the CAT model. CAs were harvested for immunohistochemistry and immunofluorescence analysis 0.5h after a 3-minute application of 5% ferric chloride to the surface of the vessel. (A)&(B). Representative photomicrographs demonstrating immunohistochemistry for GSK3β in mouse carotid artery vascular tissues. (C)&(D). CA sections were immunostained with a combination of anti-GSK3β pAbs (green) and anti-CD31 pAbs (red). Images were digitally merged, and fluorescence intensity was quantified using Image J software. Co-localization of GSK3β and CD31 was quantified using Image Pro Plus software and calculated as a Pearson's correlation coefficient (r). Data represent means±SEM (n=6). ###P<0.001 vs. sham group; **P<0.01 vs. model group.