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The coacervate in vitro controls the release of bFGF and NGF for 35 days in a steady fashion. Equal amount of bFGF and NGF were combined, then mixed with heparin followed by PEAD to form coacervate. After centrifuged, supernatant was collected. The amount of released bFGF and NGF was measured by ELISA at day 1, 4, 7, 14, 21, 28, and 35, Data were presented as means ± SD (n= 3 per group).
