FIG. 2.
Comparison of proteins synthesized from clone pCV4A in a TNT reaction with those produced in PEC-infected cells. At 24 h after PEC infection in the presence of IC, cells were [35S]methionine-labeled for 4 h, and cell lysates were prepared in RIPA buffer. RIPA results of the TNT reaction and cell lysates of PEC infection with the hyperimmune guinea pig sera (pre- and postimmunization) against PEC VPg (lanes 1 to 4), polymerase (lanes 5 to 8), capsid (VLPs) (lanes 9 to 12), or VP2 (lanes 13 to 16) are shown. The tentative final cleavage products consistent with the expected masses of PEC VPg, ProPol, and capsid are marked with arrows. The VP2 protein is indicated with an asterisk. Lane 17, radiolabeled proteins from a coupled TNT reaction of pCV4A.