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(A) Growth kinetics of PEC after transfection of RNA transcripts from pCV4A in the presence of IC (1%) or GCDCA (200 μM) by ELISA. RNA transcripts (1 μg) were transfected in LLC-PK cells with Lipofectamine 2000, and the transfected cells were incubated with IC or GCDCA for the desired period. (B) Growth kinetics of Cowden PEC and pCV4A viruses in the presence of IC (1%) or GCDCA (200 μM) by ELISA. The viruses were inoculated at an MOI of 0.5.
