TABLE 1.

RNA and RT levels in wt, H84Y, and H84A viruses^a

Construct	RNA level	RT level
wt	100	100
H84Y	94	113 ± 12
H84A	109 ± 9	106 ± 51

^aViral genomic RNA (RNA) and RT levels in wt, H84Y, and H84A viruses are expressed as percentages of the values obtained for wt viruses. RNA levels were determined by RNAse protection using RNA isolated from equivalent amounts of virus. Relative levels were quantitated densitometrically from one (H84Y) or two (wt, H84A) independent experiments. RT values are derived from three independent measurements for each construct and were determined using exogenous RT assays, normalized for capsid protein levels by densitometric quantitation from immunoblots.