FIG. 6.

EBNA1 reverses the ability of Nm23-H1 to suppress cell migration. MDA-MB-435 cells were transfected with Nm23-H1, EBNA1, or both and selected in culture. All colonies were collected and amplified, followed by analysis for expression of individual proteins by Western blotting. Cell migration assays were performed with three different chemoattractants. In each case the cells containing Nm23-H1 plus EBNA1 showed a reversal of the ability of Nm23-H1 to suppress migration. Western blots at the bottom show the signals for both EBNA1 and Nm23-H1.