FIG. 7.

Cell migration assays were performed to further address and identify the responsible domain of EBNA1 that contributes to cell migration. We obtained deletion mutants of EBNA1. MDA-MB-435 cells were transfected with Nm23-H1, the deletion mutant of EBNA1, or both and selected in DMEM containing 2 mg of neomycin/ml. A cell motility assay was performed in 0.5% fetal bovine serum (FBS) chemoattractant. The p367 mutant showed suppressing motility that is critical for EBNA1's role in reversing the ability of Nm23-H1 to suppress motility.