Table 1.

Fitting of Urea Denaturation Curves to the Two‐State Model for Protein Folding of V _H‐DF Mutant and its Elongation Mutants

Parameter	ΔG F‐U	m F‐U	[D]F‐U
Variant
V H‐DF	−21.8 ± 0.8	7.1 ± 0.2	3.1
V H‐DF/C1	−21.5 ± 1.0	7.0 ± 0.3	3.1
V H‐DF/C2	−22.2 ± 0.8	7.1 ± 0.2	3.1
V H‐DF/C3	−23.0 ± 0.7	7.3 ± 0.2	3.2

C1 (V _L‐el‐R108G), C2 (V _L‐el‐R108), and C3 (V _L‐el‐R108T109). ΔG in kJ mol⁻¹; m in kJ mol⁻¹ M⁻¹. F = folded state; U = unfolded state; [D] between states = denaturant 50% in M.