FIG. 5.
Coimmunoprecipitation of UL16 and UL21 proteins. Lysates from metabolically labeled PSEK cells infected with PrV-Ka, PrV-ΔUL16F, or PrV-ΔUL21F were precipitated with the UL16-specific antiserum (α-UL16) or the corresponding preimmune serum (α-UL16NS), the UL21-specific antiserum (α-UL21), or an anti-gH (α-gH) monospecific serum. Precipitates were separated on an SDS-10% polyacrylamide gel and analyzed with a phosphorimager. Labeled marker proteins are shown in the left lane, and their relative molecular masses are indicated.