FIG. 8.
Growth properties of mutants PrV-ΔUL16F/11G, PrV-ΔUL16F/21K, and PrV-ΔUL16F/21K/11G. (A) One-step growth kinetics. RK13 cells were infected with the indicated viruses at an MOI of 5 and harvested as described in the legend to Fig. 4. Progeny virus titers were determined on RK13 cells. Shown are means and standard deviations from three independent experiments. (B) Plaque size determination. RK13, RK13-UL11, RK13-UL16, or RK13-UL21 cells were infected with PrV-Ka, PrV-ΔUL11F, PrV-ΔUL16F, PrV-ΔUL21F, PrV-ΔUL16F/11G, PrV-ΔUL16F/21K, or PrV-ΔUL16F/21K/11G under plaque assay conditions. Plaque size was determined 2 days p.i. Mean relative values and standard deviations from three different experiments are shown, with the PrV-Ka plaque size set at 100%.