FIGURE 1.

PD-1 expression on activated regulatory T-cells (aT_reg) and CD4⁺ T-cells. a) Frequencies of aT_reg and PD-1⁺ aT_reg in peripheral blood mononuclear cells (PBMCs) and tumour-draining lymph nodes (TDLNs) (p<0.01). b) Representative dot plot showing PD-1^hi gating on activated (act)CD4⁺ T-cells in TDLNs, and frequencies of PD-1^hi actCD4 T-cells among PBMCs and TDLNs (p<0.001). c) PD-1 mean fluorescence intensity levels on aT_reg, actCD4⁺ and cytokine-secreting (cyt)CD4⁺ T-cells. Paired t-tests were performed to determine statistical significance between matched PBMCs and TDLNs: **: p<0.01.