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. 2017 Jan 5;24(2):92–103. doi: 10.1038/gt.2016.80

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Murine CD40L activates splenocyte co-cultures. Splenocytes from mice were co-cultured with murine Panc02 tumor cells (10:1) infected with LOAd(−), mLOAd700 with murine TMZ-CD40L, LOAd700 with human TMZ-CD40L or left uninfected. After 3 days culture, supernatants were collected and analyzed by Meso Scale. Background from unstimulated splenocytes is withdrawn. The experiment show duplicate samples and statistical differences were calculated using one-way analysis of variance with Dunnett's multicomparison test against control virus group (LOAd(−)) at a 95% confident interval.