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. 2017 Jan 5;24(2):92–103. doi: 10.1038/gt.2016.80

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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LOAd700-stimulated DCs expand antigen-specific T cells and NK cells. Peripheral blood mononuclear cells expanded using DCs from CMV-positive blood donors (n=3). (a) Expansion of CD3+, CD8+ and double-positive T lymphocytes of all cultured cells are shown in the left panel, and the percentage of CMV-specific T cells of all gated CD3+CD8+ T cells against forward scatter are shown in the right panel. (b) The left panel demonstrates the total number of cells in the co-cultures using 0.01 or 10ug pp65 CMV peptides. The right panel shows percentage of CMV-specific CD3+CD8+ T cells in the co-cultures using 0.01 or 10 μg pp65 CMV peptides as calculated by multiplying the % cells with the manual count of cells in the cultures. Representative figures are shown.