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FKS1 gene structure and deletion plasmid. (A) The genomic structure of the FKS1 locus is depicted, with a scale bar below in 1-kb increments. Primers used to generate PCR fragments containing the upstream and downstream flanking sequences with added restriction sites for cloning are indicated. (B) pAM1253 is the broad-host-range plasmid used to transform C. posadasii and create the FKS1 deletion.
