FIG. 5.

Transformant strains with ΔFKS1::hphB alleles show hyphal defects. (A to C) Transformant strains were grown for 72 h in 2× GYE agar medium with hygromycin at 50 μg/ml, stained with DAPI and calcofluor, and visualized using a Leica compound microscope with fluorescent optics. The strains in the micrographs are as follows: VFC12 (ΔFKS1::hphB; FKS1^WT) (A), VFC16 (ΔFKS1::hphB; FKS1^WT) (B), and VFC13 (ectopic hphB; FKS1^WT) (C). Scale bars are indicated in the upper left corners of the micrographs. Arrows indicate abnormally shaped hyphal compartments. (D to F) Transformant strains and the wild-type Silveira strain were grown as described above, with the inclusion of caspofungin (20 μg/ml) and the omission of hygromycin. The strains shown are as follows: Silveira (D), VFC13 (E), and VFC12 (F).